Question
Question: Write the dual purpose served by deoxyribonucleoside triphosphates in polymerisation....
Write the dual purpose served by deoxyribonucleoside triphosphates in polymerisation.
Solution
Deoxyribonucleoside triphosphate is the nucleotides substrates that have deoxyribose sugar which is connected to the four bases that include adenine, guanine, cytosine and thymine on its first carbon and another phosphate group which are attached to the other carbon of the deoxyribose.
Complete answer:
The dual-purpose which is served by deoxyribonucleoside triphosphate includes serving as a substrate towards the reaction and providing energy to the polymerisation reaction which is acting as an energy source. The deoxyribonucleoside triphosphate is the building blocks of DNA and is of four types that are useful in DNA replication and repair. They are important for the replication of DNA for few minutes in the eukaryotic cells and are limited in quantity due to ribonucleotide reductase in the substrates for DNA polymerizing enzymes which are the enzymes that help in the conversion of ribonucleotides to deoxyribonucleotides.
Deoxyribonucleoside triphosphates present in the eukaryotic cells are in low concentrations and perform various cell cycles. The size of the deoxyribonucleoside triphosphate gets shrunk when the cell stops cycling. The number of deoxyribonucleoside triphosphates in a cell can be detected by a sensitive and reliable quantification method which is necessary for the detection of changes and manipulation in the cells. They are identified by two different methods which are the high-performance liquid chromatography and the enzymatic assay with the DNA polymerase.
Note:
The deoxyribonucleoside consists of the monomeric units of the informational biopolymer which is the deoxyribonucleic acid. The mismatch during the process of DNA replication is due to the tautomeric forms of deoxyribonucleoside triphosphate. The energy provided by deoxyribonucleoside triphosphate is by the cleavage of high-energy terminal phosphate bonds.