Question

Which substance is used in column Chromatography?

Answer 1

In column chromatography, a stationary phase and a mobile phase are used to separate and analyze different components of a mixture. The stationary phase is typically a solid material that is packed into a column, and the mobile phase is a liquid or gas that flows through the column, carrying the mixture with it.

One common substance used as the stationary phase in column chromatography is silica gel, which is a porous form of silicon dioxide. Silica gel has a high surface area and is available in various particle sizes, making it suitable for adsorbing different components of a mixture based on their interactions with the stationary phase. Other materials like alumina can also be used as the stationary phase in column chromatography, depending on the specific requirements of the separation.

The choice of the mobile phase depends on the nature of the compounds being separated and the goals of the separation. Common mobile phases include various solvents or solvent mixtures that help elute the components of the mixture through the column.

The most commonly used adsorbent in column chromatography is Al2O3 and silica gel because these have high surface area and are porous in nature such that they absorb substances onto the surface by intermolecular forces.