Question

Which statement correctly describes sequential steps in cDNA cloning?

• A. Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector
• B. MRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase, ligation to vector
• C. MRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector
• D. Double stranded cDNA synthesis, restriction enzyme digestion, additino of linkers, ligation to vector

Answer 1

Answer: (A)

Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector