Question

Which stain is commonly used in study of cell division?
A)Feulgen reaction
B)Acetocarmine
C)Eosine
D)Safranin

Answer 1

M phase or mitotic phase is termed as the phase during which actual cell division occurs. It is observed to be the most dramatic period of the cell cycle. Before this phase, the cell duplicates its components and at the time of this phase, the distribution of the already duplicated components takes place in a well-defined manner. It is a constitution of two processes.

Complete answer:
Cell division is completed in two processes:
Karyokinesis - It is referred to as the process where the division of the nucleus Occurs.
Cytokinesis - It is referred to as the process where the division of cytoplasm occurs.
The DNA takes place in a tangled form during cell division. So, the stain is present that clearly shows the condensation of chromosomes during the prophase of mitosis.

Feulgen reaction: It is first observed by Robert Feulgen. It is utilized to observe chromosomal material and DNA in a cell. It provides a dark stain. It is utilized to stain DNA but it does not give information about condensation of chromosomes.
Acetocarmine: It is referred to as a nuclear strain that binds with chromosomes thus it is used to show condensation of chromosomes. It is a kind of dye that is mostly obtained from insects.

Eosine : Eosin is a kind of fluorescent acidic compound that connects to forms salts with basic, or eosinophilic, compounds that carry positive charges for example proteins that are positive due to the reason that they contain amino acid residues such as arginine and lysine and stains them dark red or pink because of result of the actions of bromine on fluorescein.
Safranin: It is utilised to detect gram-negative bacteria. It is a kind of basic stain that is utilized for study of histology and also cytology. It provides the nucleus with a red colour.

Hence, the correct answer is option (B)

Note: Mitogens are referred to as substances which stimulate mitosis for example: Auxin, Cytokinin, Gibberellin, Insulin. All those substances or chemicals which stop mitosis or prevent the cells from entering into normal mitotic divisions are termed as mitotic poisons. The enzyme ribonuclease plays a role as poison at prophase.