Question

Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?

• A. The emission maxima ($\lambda_{max}$)is independent of the excitation wavelength.
• B. The emission maxima ($\lambda_{max}$) depends on the concentration of a quencher.
• C. The emission maxima ($\lambda_{max}$) varies with solvent polarity.
• D. The emission maxima ($\lambda_{max}$) varies with temperature

Answer 1

Answer: (A), (C), (D)

The emission maxima ($\lambda_{max}$)is independent of the excitation wavelength.

Answer 2

The correct options are:(A):The emission maxima (max) is independent of the excitation wavelength., (C):The emission maxima (max) varies with solvent polarity,(D): The emission maxima (max) varies with temperature .