Question

Which one of the following viruses has single stranded DNA and is used extensively as a cloning vector in recombinant DNA technology?
A. TMV
B. Lambda Phage
C. Adenovirus
D. ${M_{13}}$

Answer 1

When a genetically modified vector is introduced and incorporated into the genome of an entity (host), recombinant DNA technology modifies the phenotypic of the entity (host). As a result, the procedure comprises inserting a foreign DNA fragment into the genome that contains the target gene. The introduced gene is referred to as a recombinant gene, and the procedure is referred to as recombinant DNA technology. In recombinant DNA technology, the desired gene is chosen for injection into the host, followed by the perfect vector into which the gene must be incorporated, and so recombinant DNA is created. The recombinant DNA must next be injected into the host. Finally, it must be preserved in the host and passed down to the offspring.

Complete answer:
Option A: Tobacco mosaic virus (TMV) is a positive-sense single-stranded RNA virus that infects a broad variety of plants, particularly tobacco and other members of the Solanaceae family.
So, option A is incorrect.
Option B: It's a bacteriophage, or bacterial virus, that infects the Escherichia coli bacteria.
So, option B is incorrect.
Option C: Adenoviruses are non-enveloped, medium-sized viruses with an icosahedral nucleocapsid and a double-stranded DNA genome. They infect a wide range of vertebrate hosts, and different adenovirus serotypes have been reported to cause a variety of ailments in humans, ranging from minor respiratory infections in infants to life-threatening multi-organ disease in patients with weaker immune systems.
So, option C is not correct.
Option D: It is a circular single-stranded DNA virus that is employed in DNA technology as a cloning vector.
So, option D is correct.

Hence, Option D is the correct answer.

Note:
Recombinant DNA Technology is a method for creating artificial DNA by combining genetic resources (DNA) from various sources. The presence of HIV in a person can also be detected using DNA technologies. It is used to try to fix the genetic abnormalities that cause hereditary disorders. The application of recombinant DNA technology in agriculture is widely employed to develop genetically modified species such as Flavr Savr tomatoes, golden rice rich in proteins etc. Insulin is made using recombinant DNA technology in the pharmaceutical industry.