Question
Biology Question on biotechnology
Which one of the following statements is not true regarding gel electrophoresis technique?
A
The process of extraction of separated DNA strands from gel is called elution
B
The separated DNA fragments are stained by using ethidium bromide
C
The presence of chromogenic substrate gives blue coloured DNA bands on the gel
D
Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light
Answer
The presence of chromogenic substrate gives blue coloured DNA bands on the gel
Explanation
Solution
Gel electrophoresis does not employ chromogenic substrates; instead, the DNA is made visible by intercalating it with EtBr, which can be viewed under UV light. Without UV illumination, DNA bands are not discernible in the gel.