Question

Which of the following techniques were used by Messelson and Stahl to show that DNA replication is semi-conservative?

(i) Growing E. coli in defined synthetic medium.

(ii) Use of [³H]- thymidine and analysis of the products by autoradiography.

(iii) Use of heavy nitrogen and analysis of the products by equilibrium density gradient centrifugation.

• A. (i) and (ii) only.
• B. (i) and (iii) only.
• C. (ii) and (iii) only.
• D. (i), (ii) and (iii)

Answer 1

Answer: (B)

(i) and (iii) only.