Question

Which of the following steps is/are catalyzed by Taq polymerase in a PCR?
A. Denaturation of template DNA
B. Annealing of primers to template DNA
C. Extension of primer end on template DNA
D. All of these

Answer 1

PCR is a technique used in the laboratory to create a large number of replications of a particular DNA fragment. It developed for the first time during the 1980s. PCR involves a warming and cooling mechanism called hot cycling, which is performed by a machine. Tad polymerase acts in the polymerization process.

Complete answer: In sub-atomic chemistry, PCR is shorthand for a straightforward but extremely useful process called the polymerase chain reaction. It is a mechanism used to strengthen an intrigue portion of DNA or create sections and loads of duplicates. All in all, from an initial small example, PCR allows you to create a large number of duplicates of a given DNA arrangement, in some cases even a single duplicate. For a number of inherited technologies, it is an effective method and in reality, has empowered the promotion of a set-up of new advances. The process of PCR are as follows:
Step 1: Denaturation - The two strands in the DNA double helix should be separated, just as in DNA replication. By raising the temperature of the blend, the separation takes place, allowing the hydrogen bonds between the reciprocal DNA strands to split. Denaturation is called this process.

Step 2: Annealing - Preliminaries are connected to the target groupings of DNA and begin polymerization. Once the temperature of the device has been brought down, this can only happen. One tentative link with each strand.

Step 3: Extension - Using the first strands as layouts, new DNA strands are created. Free DNA nucleotides are consolidated by a DNA polymerase catalyst. Taq polymerase, a compound initially disengaged from a thermophilic microbe named Thermus aquaticus , is a standard protein. The grouping of nucleotides into the first (layout) DNA strand controls the request in which the free nucleotides are included. Two double abandoned successions of target DNA, each containing one recently made strand and one special strand, are the result of one pattern of PCR. As most processes using PCR involve enormous quantities of DNA, the procedure is usually rehashed (typically 20-30). To get a billion or so duplicates only takes 2-3 hours. The enzyme that binds nucleotides together is Taq polymerase and helps to extend the primary end of the DNA template.

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Therefore, the right answer is option C.

Note: The accompanying developments included in the process of denaturation.
Denaturation: The double abandoned DNA is heated up to cause the hydrogen bonds to split and isolate two strands.
Strengthening: The two groundwork arrangements that link to the right correlative portion of the DNA strand are added.
Augmentation: Using the nucleotides given in the medium and using the format strand, the Taq polymerase chemical polymerizes the nucleotide chain.