Question

Which of the following is required for micro-injection method of gene transfer?

• A. Micro-particles
• B. Micro-pipettes
• C. Divalent cations
• D. $UV$ radiations

Answer 1

Answer: (B)

Micro-pipettes

Answer 2

Micro-injection method is the direct or vectorless method of gene transfer, in which foreign $DNA$ is directly injected into the nucleus of animal cell or plant cell by using micro-needles or micro-pipettes. It is used to transfer $DNA$ in oocytes, eggs and embryo.