Question
Biology Question on biotechnology
Which of the following is not a desirable feature of a cloning vector?
A
Presence of origin of replication
B
Presence of a marker gene
C
Presence of single restriction enzyme site
D
Presence of two or more recognition sites
Answer
Presence of two or more recognition sites
Explanation
Solution
In order to link the alien DNA, the cloning vector needs to have very few, preferably single recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.