Question

Which of the following is not a characteristic of $pBR322$ vector?

• A. It was the first artificial cloning vector constructed in $1977$ by Boliver and Rodriguez
• B. It is the most widely used, versatile and easily manipulated vector
• C. It has two antibiotic resistance genes $tet^R$ and $amp^R$
• D. It does not have restriction site for Sal I

Answer 1

Answer: (D)

It does not have restriction site for Sal I

Answer 2

pBR322 has a restriction site for Sal I.