Question

Which of the following are correct about EcoR1?
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognise a specific palindromic sequence
D. Cut the DNA between the base G and A when encounters the DNA sequence ‘GAATTC’
E. Exonuclease
Choose the correct answer from the options given below:

• A. B, C, E only
• B. A, D, E only
• C. A, C, D only
• D. B, C, D only

Answer 1

Answer: (D)

B, C, D only

Answer 2

EcoRI is a restriction endonuclease that recognizes the palindromic sequence GAATTC and cuts the DNA between G and A, producing sticky ends.
It does not produce blunt ends or act as an exonuclease.