Question

Which Enzyme is used in the ELISA test
(a) Hydroxylase
(b) Nuclease
(c) Alkaline phosphatase
(d) Protease

Answer 1

It is the enzyme present mainly in the liver, bones, kidneys, and digestive systems. In situations such as an injury in the liver, it spills into the bloodstreams. It is a good biochemical marker for bone formation.

Complete step by step answer:
- ELISA Test is used to diagnose HIV infection, toxoplasmosis, Lyme disease. Detection is carried out by the addition of a substrate that can generate a color.
- In the detection of Elisa many substrates are available that can be used. The most commonly used are- horseradish peroxidase (HRP) and Alkaline phosphatase (ALP) .
- For the detection, a system is formed that consists of an Alkaline phosphatase that conjugates with antibodies and uses p- nitrophenyl phosphate as the substrate that results in the production of the yellow water- soluble reaction product.
So, the correct answer is,” Alkaline phosphatase”.

Additional information:
- In the ELISA test an antigen must be immobilized to a solid surface and then complex with an antibody that is linked to an enzyme.
- Hydroxylase- are the enzymes that add a hydroxyl group to organic compounds and the process is called hydroxylation.
- Nuclease- are the enzymes capable of delivering the phosphodiester bond and play their important role in various DNA repair processes involved in DNA replication, base excision repair, nucleotide excision repair, double- strand break repair.
- Protease- help in The breakdown of protein into smaller polypeptides or single amino acids.

Note: Alkaline phosphatase is an enzyme found in the bloodstream. It helps in the breakdown of protein in the body and exists in different forms depending on where it originates. It aids in the break- down process of proteins in the body and exists in different forms. In general, Alkaline phosphatase is active in alkaline pH environments.