Question

Which enzyme is used for the separation of genetic material?
A. Ligase
B. Restriction endonuclease
C. Hydrolase
D. Amylase

Answer 1

Hint:- The enzyme is defined as the catalyst that accomplishes numerous chemical reactions at a very high rate within the favorable conditions of temperature, pH, and pressure of the cells. The genetic material is defined as that material that presents in the nucleus, mitochondria, and cytoplasm, and plays an important role in assuming the structure and behaviour of the cell and variation.

Complete answer: Ligase is defined as an enzyme that can catalyse the joining of 2 huge molecules by establishing a new chemical bond between them. An enzyme DNA ligase is known as DNA joining enzyme. Restriction enzymes are defined as the DNA-cutting enzymes present in bacteria and collected from them for use. These enzymes cut within the molecule. Hence they are known as restriction endonucleases. This enzyme recognizes and breaks DNA at a specific sequence of nucleotides. So, restriction endonucleases are utilized for separation of genetic material. In the process of DNA cloning, restriction enzymes and DNA ligase are utilized to inject genes and extra pieces of DNA inside the plasmids. Hydrolase is defined as an enzyme that catalyses the hydrolysis of a chemical bond by using water. Amylase is defined as an enzyme that catalyses the conversion of starch into sugars such as glucose as maltose. This enzyme is found in the saliva of humans and helps in digestion.
So, the correct answer is option B i.e. Restriction endonuclease.

Note:- All enzymes are assayed for work every 3-6 months. The expiration date is provided on the attached label to each vial of the enzyme. After 35 years of experience with restriction enzymes, scientists conclude that the maximum is extremely stable when stocked at -20°C in the advised storage buffer.