Question

What is the role of ori for cloning vectors?

Answer 1

The terminology ORI is the origin of replication.A cloning vector is a small part of DNA that can be stably maintained in an organism, into which a foreign DNA fragment can be inserted for cloning purposes.

Complete step by step answer:
·The cloning vector can be DNA taken from a virus it can be the cell of a higher organism or the plasmid of a bacterium. The vector contains features that allow inserting or remove a DNA fragment to or from the vector.
· A phagemid is a DNA based cloning vector, which has both bacteriophage and plasmid properties. This vector carries the origin of plasmid replication.
· The origin of replication is a particular sequence in a genome at which replication is started. Multiplication of the genetic material between generations requires accurate duplication of DNA by semiconservative replication before cell division to confirm each daughter cell receives the full complement of chromosomes.
· This can either involve the replication of DNA in the living organism.

Note: ORI is a genetic sequence that acts as an initiation site for replication of DNA any fragment of DNA when linked to the ori region, can be initiated to replicate.