Question

What is the difference between PCR and DNA sequencing?

Answer 1

The PCR and DNA sequencing are the methods from the field of Biochemistry. Both are the methods for the determination of the DNA sequences that help in the detection of various diseases if present in an individual.

Complete answer
The difference between PCR and DNA sequencing are as follows:

PCR	DNA sequencing
The Polymerase Chain Reaction is the method of making millions of DNA copies from a DNA sample.	DNA sequencing is the method of determining the sequences of the DNA.
The PCR has two primers that face each other and start their function.	Here only one primer is present in one direction and starts to read the DNA sequences in a unidirectional flow.
The DNA strands that are copied will be two.	The DNA strands that are copied will be one.
Here the DNA after amplification will then be sequenced.	Here the DNA sequencing does not undergo an amplification process.
It is mainly used for genetic fingerprinting.	It is used for several cases that involve criminal, medical, or research.
Here the DNA amplified exponentially.	Here the DNA amplifies linearly.

Additional information
-PCR is used within the early stages of processing DNA for sequencing and for detecting the presence or absence of a gene to assist in identifying pathogens during infection, and PCR is also used during generating forensic DNA profiles from tiny samples of DNA.
-The PCR requires two reagents that help the process to occur, the first one is the DNA primer which is a short DNA sample and the other is the DNA polymerase.
-In Sanger sequencing, the DNA sample to be sequenced is combined during a tube with primer, DNA polymerase, and DNA nucleotides (dATP, dTTP, dGTP, and dCTP).

Note:
The polymerase chain reaction (PCR) was originally developed by the American biochemist Kary Mullis in 1983. In 1993, he was awarded the Nobel Prize in Chemistry for his work. Sanger sequencing gives high-quality sequences for relatively long stretches of DNA (up to about 900 base pairs). It typically won’t sequence individual pieces of DNA, like bacterial plasmids or DNA copy.