Question

What is micro-injection?

Answer 1

Microinjection is the injection which is used to inject a liquid substance or any other substance at a microscopic level, and the injecting cell is often a living cell and it may also include the intercellular space, and this process also involves the use of an inverted microscope with a magnification power around 200x.

Complete answer:
- Generally, this microinjection is used to inject the recombinant DNA, in the nucleus of the target cells.
- For this process to happen, the target cell should be placed under the microscope and two micromanipulators are required where one is used for holding pipette and one is used for holding microcapillary needle usually between the range of 0.5 to 5 micrometers, which is used to penetrate the cell membrane and also nuclear envelope.
- By using this microinjection, a vector can be introduced into a single cell.
- Micro infection can also be used in the cloning of organisms in the study of cell biology and viruses.
- There are two basic types of the micro injection system, where the first one is called a constant flow system, which is very simple and inexpensive, wherein this system there is a constant flow of samples delivered.
- The second type of microinjection is called Pulsed flow, and this system has great control and consistency over the amount of sample injecting.
- There is another technique called pronuclear microinjection, in These genes are directly injected into the nucleus of the oocyte, where it is produced by transgenic organisms.

Note: This is a process where one can inject the substance in cells that are at a microscopic level, and this can be done by using a micropipette, where the injection of organisms is done by piercing the cell membrane or directly by piercing the nuclear envelope.