Question

What is meant by gene gun method?

Answer 1

The gene distribution method requires the use of the insertion into host cells of foreign genetic material, such as DNA or RNA. To cause gene expression, the genetic material must enter the host cell's genome.

Complete answer:
A gene gun or biolistic particulate delivery system is a mechanism used in genetic engineering to transfer exogenous DNA (transgenes), RNA, or protein to cells. The incorporation of desired genetic material can be triggered into cells by covering particles of heavy metal with a gene of interest and shooting these micro-projectiles into cells using mechanical energy. Biolistics is also referred to as the process concerned with such micro-projectile delivery of DNA

Biolistic transition entails the incorporation into target cells of a functional fragment of DNA, known as a DNA build. A gene construct is a DNA cassette containing all appropriate regulatory elements for proper expression within the target organism. Although gene constructs may differ based on the desired outcome of the transformation process in their nature, all constructs usually contain a combination of a sequence of promoters, a sequence of terminators, the gene of interest, and a gene of reporters

Note: Biolistics has proved to be a flexible genetic engineering tool and transformation-resistant crops, such as cereals, are typically favored to be engineered. In particular, Bt maize is a consequence of biolistics. Compared to other existing strategies, such as Agrobacterium mediated transformation, which have difficulty targeting the vector to and stably expressing in the chloroplast, plastid transformation has also seen great success with particle bombardment.