Question

What is/are the use(s) of thin layer chromatography?
A. To monitor the progress of a reaction
B. To identify compounds present in a given mixture
C. To determine the purity of a substance
D. All of the above

Answer 1

We have to know that thin layer chromatography (TLC) is a usually utilized strategy in engineered science for recognizing compounds, deciding their virtue and following the advancement of a reaction. It additionally allows the enhancement of the solvent system for a given separation issue. In examination with column chromatography, it just requires little amounts of the compound (~ng) and is a lot quicker too.

Complete answer:
We have to know that thin Layer Chromatography is a method used to segregate non-volatile mixtures. The analysis is directed on a sheet of aluminum foil, plastic, or glass which is covered with a thin layer of adsorbent material. The material typically utilized is aluminum oxide, cellulose, or silica gel.
On the finish of the partition, every segment shows up as spots isolated upward. Each spot has a maintenance factor (Rf) communicated as:
${{\text{R}}_{\text{f}}}{\text{ = }}\dfrac{{{\text{Distance travelled by sample}}}}{{{\text{Distance travelled by solvent}}}}$
The elements influencing the hindrance factor are the solvent system, measure of material spotted, permeable and temperature. We have to know that this chromatography is one of the quickest, most economical, least difficult and simplest chromatography procedures.
Thin layer chromatography (TLC) relies upon the principle of separation. The partition depends on the overall liking of mixtures towards both the stages. The mixtures in the mobile phase move over the outside of the stationary phase. The development happens so that the mixtures which have a higher partiality to the stationary phase move gradually while different mixtures travel quickly. Consequently, the separation of the mixture is accomplished. On consummation of the division cycle, the individual components from the mixture show up as spots at separate levels on the plates. Their character and nature are recognized by a reasonable detection process.
We can use thin layer chromatography to observe the progress of reaction and also to obtain its purity.

So, the correct answer is “Option D”.

Note:
We have to know that some of the applications of thin layer chromatography are in cosmetic industry, to know if the reaction is complete, used in biochemical analysis, qualitative testing of several medicines like anticonvulsant, tranquilizers, used in food industry to segregate sweetening agents, preservatives etc.