Question

What are the requirements in Tissue culture?
A. Hormones like auxin, cytokinin, agar-agar
B. Inorganic salt, vitamin, amino acid only
C. Carbon source like sucrose only
D. All of these

Answer 1

Tissue culture describes a process in which a plant or animal tissue segment is incorporated into a new, artificial environment where it continues to function or grow. Even though tissue fragments are used, it is important to realize that the entire organ is still used for purposes of tissue culture.

Complete answer:
Due to its wide range of applications, tissue culture is of great significance in biotechnological applications. The tissue culture procedures may be complex, requiring a lot of assistance to prevent certain effects such as contamination. It may not be an exercise for all, because of complexities that may be found in some of the procedures. The basic requirements of tissue culture include hormones such as auxin, cytokinin, gibberellins, potassium, calcium, and other macronutrients and macro elements, inorganic salts, vitamins, micronutrients, amino acids, carbon sources such as sucrose, glucose, plant growth regulators. Supplements like polyamines, activated charcoal, undefined supplements such as corn milk are also required for the tissue culture. Gelling agents like agar, agarose are used since they withstand sterilization processes.

So, the correct option is (D).

Note: There are several tissue culture techniques utilized as different types of tissues that require special circumstances to yield desired results for the culture process. For cloning purposes, genetic modification of a particular crop, or merely to speed up or increase the yield of the plant of interest, plant tissue culture could be used.