Question

What are the assumptions made during the calculation of net gain of ATP?

Answer 1

ATP is not even a chemical energy storage compound; that's the role of sugars, like fats, and glycogen. It is transformed from storage compounds into ATP when energy is required by the cell.

Complete answer:
First we should know about cell walls to answer this question. The energy-carrying molecule adenosine triphosphate (ATP) is present in the cells of all living organisms. ATP collects and emits chemical energy gained from the degradation of food molecules to fuel certain cellular processes. For three general types of activities, cells require chemical energy: to drive biochemical processes which do not happen automatically; to move the substances required across membranes; and do mechanical activity, like moving muscles.

Various predictions can be made for the theoretical measurement of ATP molecules, which are as continues to follow:
(a) It is believed that, in a concurrent and ordered manner, separate parts of aerobic respiration like TCA cycle, glycolysis, and ETS exist.
(b) In order to undertake oxidative phosphorylation, NADH generated during the glycolysis process reaches the mitochondria.
(c) The only substrate is known to be a glucose molecule, whereas no other molecule is thought to join the pathway at the intermediate levels.
(d) In some other method, the intermediates formed throughout respiration are not used.

Note: By moving a phosphate group to a separate molecule, ATP is sufficient to activate cellular processes. This transition is taken out by unique enzymes that pair the production of ATP energy with cellular activities requiring energy.