Question

What are gram variable bacteria?

Answer 1

Gram staining, often known as Gram's method, is a staining method for distinguishing and classifying bacteria into two broad groups: gram-positive bacteria and gram-negative bacteria. Hans Christian Gram, a Danish bacteriologist who invented the procedure, gave it his name.
The chemical and physical features of bacteria's cell walls are used to differentiate them using Gram staining. The principal stain, crystal violet, is retained by a thick coating of peptidoglycan in the cell wall of Gram-positive cells. Gram-negative cells have a weaker peptidoglycan coating, which allows ethanol to wash out the crystal violet. The counterstain, often safranin or fuchsin, turns them pink or red.

Complete answer:
There are a few bacterial species that can't be classified as gram-positive or gram-negative. This is due to the fact that these bacteria appear as a combination of pink and purple cells when stained with gramme stain. As a result, they are categorised as gram-variable bacteria.
Organisms that are gram-variable can't be classified as either negative or positive. The presence of organisms that stain gram-positive or gram-negative indicates the presence of organisms in the smear. These organisms may or may not be pathogenic. To evaluate the results, more identification is required.

Additional information:
The cell wall is the non-living, outermost portion of the cell. It's only found in plant cells, but it's also found in fungus, bacteria, and algae. The cell wall is a thick, stiff, semi-permeable membrane with elastic qualities that allow the cell wall to thicken with time. Because it lacks receptors, the cell wall is metabolically inactive and is primarily involved in:
Providing the cell with a defined shape.
Protects the cell from the outside world.
Assists in the separation of the exterior and interior environments.

Note:
Gram-positive bacteria belong to the phylum Firmicutes and are classified as a genus of bacteria. The colour of the crystal violet stain used in gramme staining is retained in these bacteria. Gram-positive bacteria appear purple in colour when examined under a microscope, indicating that they are positive in the Gram stain test. e.g. Clostridium , Mycobacterium , Actinomyces.
Gram negative bacteria are a type of aerobic bacteria that do not retain the crystal violet dye during the Gram staining method and look pink when viewed under a microscope.