Question

Under which of the following conditions will there be on the change in the reading frame of the following mRNA? 5’ AACAGCGGUGCUAUU3’.
A. Insertion of G at 5th position.
B. Deletion of G from 5th position.
C Insertion of A and G at 4th and 5th position respectively.
D. deletion of GGU from 7th, 8th and 9th position.

Answer 1

mRNA is the messenger RNA which is a single-stranded frame of an RNA molecule which corresponds to its genetic sequence of a particular DNA segment. This Sequence of mRNA forms during the transcription process when the DNA duplex is formed.

Complete answer:
Transcription is processed when an RNA sequence is formed from the DNA segment, The mRNA is a messenger RNA molecule which codes for the protein and helps in protein formation during the translation process outside the nucleus. mRNA carries codon sequence which is formed by the combination of three nitrogenous base pairs, such three nitrogenous base pair codons are responsible for the formation and arrangement of particular amino acids in a particular sequence of the chain of the protein molecule. All these things carry out by specific enzymes and RNA molecules, in the translation process tRNA called transfer RNA brings the amino acid molecule over the chain of mRNA and attached to the growing chain of amino acid, which undergoes the further process for the formation of protein.

The change in the codon of the mRNA sequence will lead to the mutation, which will harm the protein sequence by creating an altered protein product, which may be functional or may not be functional.

The mutation will occur by insertion of any base pair in the mRNA strand or by deletion or by the improper arrangement of base pair in the sequence of mRNA.

So that the given sequence of mRNA strand, if the deletion of GGU from 7th, 8th and 9th position, does not cause any change in the reading frame of mRNA sequence.
Hence, the correct answer is option (D).

Note: To avoid the mutation in the sequence of mRNA frame, eukaryotic pre mRNA undergoes processing, this processing takes place before moving to the cytoplasm. The main processing steps are splicing, 5’ cap addition, editing and polyadenylation.