Question

Transgenic animals are produced by injecting foreign gene into the:
A) Egg
B) Nucleus of unfertilized egg
C) Nucleus of fertilized egg
D) Nucleus of sperm

Answer 1

A transgenic animal is an animal which has a foreign DNA. The foreign gene with the assistance of vector DNA is embedded or inserted into the desired organism. This hybrid DNA is called recombinant DNA and this technique is called Recombinant DNA technique.

Complete answer:
Recombinant DNA (rDNA) molecules are DNA shaped by laboratory techniques for hereditary and genetic recombination, (for example, molecular cloning) to unite hereditary material from various sources, making sequences that would not in any case be found in the genome. There are two techniques for delivering transgenic animals, for example, transforming embryonic stem cells (ES cells) filling in tissue culture with the ideal DNA and infusing the desired gene into the pronucleus of a treated and fertilized egg of the animal.
Recombinant DNA technology involves following steps:
Step 1: To identify and isolate the gene of interest.
Step 2: To construct a recombinant DNA using a cloning vector.
Step 3: To introduce the vector in a host.
Step 4: To select the transformed recombinant cells with the gene of interest.
Step 5: To multiply and express the gene of interest.

A transgenic animal is one whose genome has been adjusted by the exchange of a gene or genes from another species or breed. Transgenic creatures are regularly utilized in the lab as models in biomedical exploration. More than 95 % of those utilized are hereditarily adjusted rodents and mice. They are significant for investigating human infection, being utilized to comprehend gene function with context to sickness defenselessness and disease susceptibility, progression and to decide reactions to a remedial intercession.

Therefore, the correct answer is option C, i.e. Nucleus of fertilized egg.

Note: Recombinant DNA technology has additionally demonstrated imperative to the creation of immunizations and protein treatments and therapies, for example, human insulin, interferon and human growth hormone. It is additionally used to create clotting factors for treating hemophilia and in the improvement of quality treatment.