Question

To confirm ELISA for AIDS which technique was used?
A. Western blotting
B. Northern blotting
C. Southern blotting
D. Eastern blotting

Answer 1

The human immunodeficiency virus (HIV) causes acquired immunodeficiency syndrome (AIDS), a chronic, potentially fatal disease (HIV). HIV impairs your body's ability to fight infection and disease by destroying your immune system.

Complete answer:
Engvall and Perlmann first described the enzyme-linked immunosorbent test in 1971, and it is now a widely used analytical biochemistry technique. The assay uses antibodies directed against the protein to be measured to detect the presence of a ligand in a liquid sample using a solid-phase type of enzyme immunoassay.
ELISA stands for enzyme-linked immunosorbent assay, and it's used to test antibodies, antigens, proteins, and glycoproteins in biological materials The capture antibody is an antigen-specific antibody produced against the target antigen.
The premise of ELISA is that particular antibodies bind to the target antigen and detect the presence and quantity of antigen binding. The plate must be coated with high affinity antibodies to maximise the sensitivity and precision of the experiment.
A healthcare provider's first test order is usually an ELISA test. In the event that this test yielded a positive result, the ELISA test was followed by a Western blot to confirm the diagnosis.
The first is Western Blotting, which uses antibodies against viral antigens to detect them. In our case, a positive Western Blot indicates the presence of viral antigen, which often suggests active virus. It's possible that the patient is suffering from a viral illness.

Note: If the patient has antigen (Western Blot), the infection is most likely still ongoing. And if the patient has antibodies (ELISA), it's likely that he or she has had the virus recently, but that the infection is no longer active.