Question

The $Ti$ plasmid, is often used for making transgenic plants. The plasmid is found in

• A. Azotobacter
• B. Rhizobium of the roots of leguminous plants
• C. Agrobacterium
• D. Yeast as a $2 \,\mu m$ plasmid.

Answer 1

Answer: (C)

Agrobacterium

Answer 2

Agrobacterium tumefaciens is the causative agent of crown gall, an important disease of many commercial crops, such as raspberries. This disease has come to be recognized in recent years as being caused by a DNA plasmid (Ti plasmid) carried by bacterium and transferred to the plant cells. Following the discovery of the relationship between crown gall and the (Ti plasmid), this plasmid has come to be widely used in plant genetic engineering as a vector, novel plant genes being spliced into the host plant cells.