Question

The RNA primer is used in
A)Translation
B)Replication
C)Conjugation
D)Transformation

Answer 1

A primer is defined as a short chain of nucleic acid that allows sequence synthesis as a starting point. They are structured in an order that the target region (the region that should be copied) is flanked. That is, sequences that will help to carry them to bind to opposite strands of the template strand are given.

Complete answer:
For a PCR reaction, to carry out a DNA primer is not needed. The non-availability and absence of DNA primers is the reason why RNA primers should be used in PCR. The DNA Primase enzyme, which is none other but RNA polymerase much like mRNA, synthesises the RNA primers complementary to the cellular DNA.

Now let us find solution from given options:
Translation : It is defined as the process of the formation of the amino acids from the RNA. In other words, translation is the polymerization of the amino acids to give rise to a polypeptide chain. The sequence and the order of the amino acids are controlled by the sequence of the bases that are located in the messenger of the mRNA.
Replication : Replication is defined as the process of the development of a new strand of DNA from an old one that is known as the parental or the template DNA. RNA primer plays a major role in this process.
Conjugation : Conjugation is an important method that is used in DNA recombination, which works in connection with transformation and transduction.
Transformation : Transformation and transduction are the two major processes that can be performed to transfer DNA matter from one bacterial cell to another.

Hence, the correct answer is option (B)

Note: The main and major difference between replication and transcription is that during replication the overall. DNA gets copied or replicated whereas, during transcription only a segment of the DNA is replicated. Also, during replication, both the strands of the DNA behave as a template strand whereas, in transcription only one strand is the template strand.