Question

The given figure is the diagrammatic representation of the E. coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)?

• A. ori-original restriction enzyme
• B. rop-reduced osmotic pressure
• C. Hind III, EcoRI - selectable markers
• D. amp $^R$, tet $^R$- antibiotic resistance genes

Answer 1

Answer: (D)

amp $^R$, tet $^R$- antibiotic resistance genes

Answer 2

In pBR 322, ori-represents site or origin of replication rop-codes for proteins that take part in the replication of plasmid. Hin d III, Eco RI- recognition sites of restriction endonucleases. amp and tet - antibiotic resistance genes.