Question

The following diagram shown restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’gens :

• A. The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid.
• B. The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.
• C. The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics
• D. Gene ’X’ is responsible for recognitions sites and ‘Y’ is responsible for antibiotic resistance

Answer 1

Answer: (B)

The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.

Answer 2

In the given diagram, ’X’ represents ori and ’Y’ represents rop:

1. ’X’ is ori (Origin of Replication):

The ori is responsible for controlling the copy number of the linked DNA. It is the start ing point for plasmid replication within the host cell.

2. ’Y’ is rop (Replication of Plasmid):

The rop protein regulates plasmid replication by stabilizing the ori region. This ensures proper replication and maintenance of plasmids within the host cell.

- ’X’ is ori and controls the copy number of the linked DNA.

- ’Y’ is rop, which codes for a protein involved in the replication of plasmids.