Question

The first report of successful production of haploid plants from ‘anther culture’ in Datura innoxia, came from the work of
A) Steward and White
B) Nitzeli and White
C) Skoog and Murashige
D) Guhe and Maheshwari

Answer 1

The advantages include high haploid plant frequencies, simple to induce cell division in certain species and no high level of expertise needed. It includes choosing appropriate parents and performing a cross in a breeding system, based on the targets.

Complete answer: To solve this question, first we should know about plant’ cell wall. Tissue culture is the growth of tissues or cells isolated from the parent organism in an artificial medium.
The technique is also known as micropropagation. Usually this is encouraged by using a mixture of liquid, semi-solid, or solid growth such as broth or agar.

Now lets us find the solution from the given options-
Through the 1940s, in the work of the American anthropologists Leslie A. White and Julian H. Steward, neo evolutionary anthropological theory arose. So, Steward and White is an incorrect answer. Thus, option A is an incorrect option.Anther culture technique has been in use for more than 30 years since Guha and Maheshwari released their first paper on anther culture in Datura innoxia. So, Nitzeli and White is an incorrect answer. Thus, option B is an incorrect option.
Guha and Maheshwari first recorded anther culture in the 1970s by in vitro methods. So, Skoog and Murashige is an incorrect answer. Thus, option C is an incorrect option.

Additional information: Pollen culture is a technique for the development of haploid plants by cultivating pollen grains collected from a plant's anther under the aseptic condition on known composition artificial media. Guha and Maheshwari also discovered the technique. So, Guha and Maheshwari is the correct answer.

Thus, the option D is the correct option. i.e. Guha and Maheshwari

Note: The pollen's were collected by pressing the anthers with hypochlorite after surface sterilization. Pollen collected has been grown on nutrient media.