Question

The correct order of steps in Polymerase Chain Reaction $(PCR)$ is

• A. Denaturation, Annealing, Extension
• B. Extension, Denaturation, Annealing
• C. Denaturation, Extension, Annealing
• D. Annealing, Extension, Denaturation

Answer 1

Answer: (A)

Denaturation, Annealing, Extension

Answer 2

This technique is used for making multiple copies of gene (or $DNA$ ) of interest in vitro.
Each cycle has three steps
(i) Denaturation
(ii) Primer annealing
(iii) Extension of primer