Question

Take a living tissue, grind it in trichloroacetic acid using pestle and mortar, and then strain it, you would obtain two fractions: acid-soluble and acid-insoluble fraction. Acid-insoluble fraction does not contain

• A. polysaccharides
• B. nucleic acids
• C. lipids
• D. flavonoids and alkaloids

Answer 1

Answer: (D)

flavonoids and alkaloids

Answer 2

The acid-soluble pool represents roughly the cytoplasmic composition whereas the macromolecules from cytoplasm and organelles become the acid-insoluble fraction. Together they represent the entire chemical composition of living tissues of organisms or cellular pool. All the compounds which are found in the acid-soluble pool, have their molecular weights ranging from approx. $18$ to around $800$ daltons $(Da)$. The acid-insoluble fraction, has only four types of organic compounds i.e., proteins, nucleic acids, polysaccharides and lipid.