Question
Biology Question on human reproduction
Southern blotting is:
A
(A) Attachment of probes to DNA fragments.
B
(B) Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet.
C
(C) Comparison of DNA fragments to two sources.
D
(D) Transfer of DNA fragments to electrophoretic gel from cellulose membrane.
Answer
(A) Attachment of probes to DNA fragments.
Explanation
Solution
Explanation:
Southern blotting is the attachment of probes to DNA fragments. Southern blotting is a laboratory technique used to detect a specific DNA sequence in a blood or tissue sample. A restriction enzyme is used to cut a sample of DNA into fragments that are separated using gel electrophoresis. The DNA fragments are transferred out of the gel to the surface of a membrane.Hence, the correct option is (A).