Question
Question: Some of the steps involved in the production of humulin are given below. Arrange them in the correct...
Some of the steps involved in the production of humulin are given below. Arrange them in the correct sequence and select the correct option.
(i) Synthesis of gene (DNA) for human insulin artificially
(ii) Culturing recombinant E.coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E.coli
(vi) Extraction of recombinant gene product from E.coli
A
(i), (iv), (v), (ii), (vi), (iii)
B
(ii), (i), (iv), (iii), (v), (vi)
C
(i), (iii), (v), (vi), (ii), (iv)
D
(iii), (v), (ii), (i), (vi), (iv)
Answer
(i), (iv), (v), (ii), (vi), (iii)