Question

Select the correct sequence of steps in DNA fingerprinting involving southern blot hybridization using radiolabelled VNTR as a probe. I. $\quad$Hybridization using labelled VNTR probe. II. $\quad$Isolation of DNA. III. $\quad$Transferring (blotting) of separated DNA fragments to synthetic $\quad$ $\quad$membranes, such as nitrocellulose or nylon. IV.$\quad$ Detection of hybridized DNA fragments by autoradiography. V. $\quad$Separation of DNA fragments by electrophoresis. VI. $\quad$Digestion of DNA by restriction endonucleases.

• A. I, V, VI, II, III and IV
• B. II, VI, V, IJI, I and IV
• C. V, I, VI, III, IV and II
• D. II, I, V, VI, IV and III

Answer 1

Answer: (B)

II, VI, V, IJI, I and IV

Answer 2

Answer (b) II, VI, V, IJI, I and IV