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Question: Restriction endonuclease enzyme acts upon DNA and cut it upon at specific sites. These fragments of ...

Restriction endonuclease enzyme acts upon DNA and cut it upon at specific sites. These fragments of DNA are separated by …1…. . Later on these fragments are stained by …….2…… and observed under …..3…… rays. These fragments occur as bright ….4….. Colored bands. These bands are obtained by the process of ….5….. and are used to construct recombinant DNA.

A

1-Sedimentation, 4-Blue

B

2-Fuelgen stain, 3-Gamma

C

2-Ethidium bromide, 5-Elution

D

3-Gamma, 4-Orange

Answer

2-Ethidium bromide, 5-Elution