Question

Recombinant DNA technology involves several steps during which the initial step is of isolation of DNA.which enzymes are utilized in the method for the breakdown of the fungal cell, plant cell, and bacterial cell?
(A)Lysozyme, lipases, trypsin
(B)Chitinase, cellulose, lysozyme
(C)Chitinase, cellulose, trypsin
(D)Trypsin, lipase, cellulose

Answer 1

Processes of recombinant deoxyribonucleic acid technology involves isolation of DNA of the specified DNA fragment. to chop the DNA with restriction enzymes, it must be in pure form, free from other macromolecules.

Complete answer:
Recombinant DNA, molecules of DNA from two different species are inserted into several organisms to supply new genetic combinations that are useful to science, medicine, agriculture, and industry. The DNA sequences utilized in the development of recombinant deoxyribonucleic acid molecules can originate from any species. For instance, plant DNA could also be joined to bacterial DNA, or human DNA could also be joined with fungal DNA. Also, DNA sequences that don't occur anywhere in nature could be created by the chemical synthesis of DNA and incorporated into recombinant molecules. The DNA is enclosed within the membranes, we've to interrupt the cell hospitable release DNA alongside other macromolecules like RNA, proteins, polysaccharides, and also lipids. This will be achieved by treating the bacterial cells/plant or tissue with enzymes like lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). The RNA is often removed by treatment with ribonuclease whereas proteins are often removed by treatment with protease.
Thus, the correct option is (B)Chitinase, cellulose, lysozyme.

Note: Recombinant DNA technology is the joining together of DNA molecules from two several species. The recombinant DNA molecule is inoculated into a host organism to develop new genetic combinations that are of significance to science, medicine, agriculture, and industry.