Question
Biology Question on Genetic Engineering and Biotechnology
Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:
A
Hind II, Hind II
B
Hind II, Alu I
C
Hind II, EcoR I
D
Hind II, BamHI
Answer
Hind II, Hind II
Explanation
Solution
To create a recombinant DNA molecule, both the vector DNA and the source DNA need to be cut with the same restriction enzyme. This creates complementary sticky ends that can anneal (base pair) with each other, allowing the source DNA to be inserted into the vector. If different restriction enzymes are used, the sticky ends will not be complementary, and the recombinant DNA molecule cannot be formed.