Question

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. A. Insertion of recombinant DNA into the host cell. B. Cutting of DNA at specific location by restriction enzyme. C. Isolation of desired DNA fragment. D. Amplification of gene of interest using PCR.

• A. B, D, A. C
• B. B C, A, B, D
• C. B, C, D, A
• D. C, B, D, A

Answer 1

Answer: (D)

C, B, D, A

Answer 2

The formation of recombinant DNA follows a specific sequence of steps. First, the desired DNA fragment (gene of interest) is isolated (C). Then, this DNA fragment, along with the vector DNA, is cut at specific sites using restriction enzymes (B). If the quantity of the isolated gene fragment is insufficient for subsequent steps, it is amplified using Polymerase Chain Reaction (PCR) (D). Finally, the recombinant DNA, formed by ligating the amplified gene into the vector, is introduced into a host cell (A).