Question
Chemistry Question on polymers of commercial importance
Polyethylene glycol method is used for
A
biodiesel production
B
seedless fruit production
C
energy production from sewage
D
gene transfer without a vector.
Answer
gene transfer without a vector.
Explanation
Solution
Direct gene transfer is the transfer of naked DNA into plant cells, but the presence of a rigid plant cell wall acts as a barrier to uptake. Thus, protoplasts are the favored target for direct gene transfer. Polyethylene glycol-mediated DNA uptake is a direct gene transfer method that uses the interaction between PEG, naked DNA, salts and the protoplast membrane to affect the transportation of DNA into the cytoplasm.
- Plant cells have rigid cell walls that inhibit the process of gene transfer.
- There are various techniques to transform plant cells when they are in the protoplast stage.
- Before the protoplast regenerates the cell wall, direct gene transfer is carried out.
- PEG method is an efficient method of transforming plant cells by direct gene transfer.
- PEG transfer stimulates uptake of liposomes and improves the efficiency of electroporation.
- PEG transformation is preferred over electroporation in monocot protoplasts as it has comparatively higher survival rates post-treatment.