Question

Palladium or gold salts are used in the staining section for
A. Superior optical microscope
B. Electron microscope
C. Ultraviolet microscope
D. Interference microscope

Answer 1

Staining is a technique used in microscopy which involves use of stains or dyes to enhance the contrast of microscopic images. It is used in biology and medicine in viewing the tissues and cells under different kinds of microscope.

Complete Answer:
To solve this question, we must know about different types of microscope.
Microscope is the instrument which is used to see objects which are difficult to see through naked eye. It involves the combination of lens. The light microscope is the simplest microscope. With the advancement in technology, different sophisticated microscopes have been evolved like-
1. Superior optical microscope - Fluorescent dyes are used along with iodine in the optical microscope. Osmium salts are also used.
2. Electron microscope - The salts of heavy metals like Uranium and lead are used in electron microscopy. In SEM or scanning electron microscopes, gold or palladium alts are used for staining.
3. Ultraviolet microscope - The UV light is used instead of normal light. It used fluorescent dyes for staining.
4. Interference microscope - It involves the use of fluorescent dye.

Thus the correct answer is (B).

Note: In electron microscope beam of electron is used in place of light. They have greater magnification as the wavelength of the electron is smaller than that of visible light. An electron microscope magnifies images 1000, 000X times. It is used to determine the structure of complexes, 3D structure of cells and tissues.