Question

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complementary DNA in a clone of cells, followed by its detection using autoradiography because:

• A. mutated gene does not appear on photographic film as the probe has complementarity with it.
• B. mutated gene does not appear on photographic film as the probe has complementarity with it.
• C. mutated gene completely and clearly appears on a photographic film.
• D. mutated gene does not appear on a photographic film as the probe has no complementarity with it.

Answer 1

Answer: (D)

mutated gene does not appear on a photographic film as the probe has no complementarity with it.

Answer 2

In order to detect a specific mutated gene using radioactive probes and autoradiography, the probe must be complementary to the target gene's sequence. If the probe has no complementarity with the mutated gene, it will not bind to the target gene, and therefore, it will not appear on the photographic film through autoradiography.

So, the correct option is (D): mutated gene does not appear on a photographic film as the probe has no complementarity with it.