Question

In vitro clonal propagation in plants is characterized by

• A. PCR and RAPD
• B. northern blotting
• C. electrophoresis and HPLC
• D. microscopy.

Answer 1

Answer: (A)

PCR and RAPD

Answer 2

Clonal propagation represents the technique in which vegetative tissue is used to produce plants that are genetically identical to their parents. It provides rapid vegetative multiplication of plant material for agriculture, horticulture and forestry. It can be characterized by PCR and RAPD. The polymerase chain reaction (PCR) technique, generates microgram (pg) quantities of DNA copies (upto billion copies) of the desired DNA (or RNA) segment, present even as a single copy in the initial preparation, in a matter of few hours. RAPD stands for Random Amplification of Polymorphic DNA. It is a type of PCR, but the segments of DNA that are amplified are random. No knowledge of the DNA sequence for the i targeted gene is required, as the primers will bind somewhere in the sequence, but it is not certain exactly where. Its resolving power is much lower than targeted