Question

In the PCR technology the DNA segment is replicated over a billion times. This repeated replication is catalyzed by the enzyme.
A. DNA Polymerase
B. Taq polymerase
C. DNA dependent RNA polymerase
D. Primase

Answer 1

In medical and biological research laboratories, PCR is a common tool. It is used for sequencing, for detecting the presence or absence of a gene in the early stages of processing DNA to help identify pathogens, during infection, and when producing forensic DNA profiles from tiny DNA samples.

Complete answer:
The concepts behind any PCR, regardless of the DNA sample, are the same. In order to set up a PCR, five core 'ingredients' are required-
The DNA template to be copied
Primers, short stretches of DNA which activate the PCR response
Nucleotide bases of DNA
Taq polymerase enzyme to be added to the current bases of DNA
To ensure the correct conditions for the reaction, buffer.
Taq polymerase, named after the thermophilic bacterium Thermus aquaticus, is a thermostable DNA polymerase. Its name is sometimes shortened to Taq Pol or simply Taq. It is also used in polymerase chain reaction (PCR), a process that significantly amplifies the amount of short DNA segments. T. Aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase has been recognized as an enzyme capable of withstanding the high temperature (protein-denaturing) conditions needed during PCR. The DNA polymerase from E. was therefore replaced. Initial use of coli in PCR. Taq's optimum temperature for activity is 75–80 °C, with a half-life of greater than 2 hours at 92.5 °C, 40 minutes at 95°C and 9 minutes at 97.5 °C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.

Hence, the correct answer is (B) Taq polymerase

Note: PCR is a laboratory method used to create multiple copies of a DNA segment. PCR is very accurate, and a particular DNA target from a mixture of DNA molecules may be used to amplify or copy. First, to bind to the start and end of the DNA target, two short DNA sequences called primers are planned. The DNA template containing the target is then attached to a tube containing primers, free nucleotides, and an enzyme called DNA polymerase to conduct PCR, and the mixture is put into a PCR system.