Question

In the PCR machine, which of the following is used to produce multiple copies of DNA?
A. Agrobacterium
B. Escherichia coli
C. Thermus aquaticus
D. None of the above

Answer 1

In medical and biological research laboratories, PCR is a popular method. In the early stages of DNA sequencing processing, it is used to diagnose the presence or lack of a gene to better identify pathogens during infection.

Complete Answer:
The thermal cycler is a laboratory device most often used for the polymerase chain reaction (PCR) to amplify segments of DNA. It's also known as a PCR machine or thermocycler. To render several copies of tiny pieces of DNA or a chromosome, PCR is used in molecular biology. It is possible to make thousands to millions of copies of a single segment of DNA from a very small volume of DNA using PCR.

Thermus aquaticus, one of many thermophilic bacteria belonging to the Deinococcus Thermus genus, is a species of bacteria which can withstand high temperatures. In the polymerase chain reaction (PCR) DNA amplification technique, Thermus aquaticus is the source of the heat-resistant enzyme Taq DNA polymerase, which is one of the most important enzymes in molecular biology.

The key factors that make Thermus aquaticus suitable for DNA sequencing are that it is involved in a wide range of temperatures. It is able to withstand the required protein denaturation during PCR in order to be able to automate PCR cycles. The DNA polymerase of the bacteria is very stable at high temperatures, which means it can tolerate the temperatures required in the denaturing stage of PCR to break the DNA strands apart.

So the correct option is C, Thermus aquaticus.

Note: In general, Thermus aquaticus has a cylindrical shape with a diameter of between 0.5 μm and 0.8 μm. Thermus Aquaticus has demonstrated many potential morphologies in various cultures. The rod-shaped bacteria appear to be aggregated.