Question

In the following palindromic base sequence of DNA, which one can be cut easily by a particular restriction enzyme?

• A. 5’ GATACT 3’ ; 3’ CTATGA 5’
• B. 5’ GAATTC 3’ ; 3’ CTTAAG 5’
• C. 5’ CTCAGT 3’ ; 3’ GAGTCA 5’
• D. 5’ GTATTC 3’ ; 3’ CATAAG 5’

Answer 1

Answer: (B)

5’ GAATTC 3’ ; 3’ CTTAAG 5’

Answer 2

The sequence 5' GAATTC 3' ; 3' CTTAAG 5' exhibits palindromic properties, making it easily cleavable by a restriction endonuclease. Conversely, the other sequences are not palindromic. Notably, the sequence 5' GAATTC 3' ; 3' CTTAAG 5' is recognized by the restriction enzyme EcoRI.