Question

In recombinant $DNA$ technology, the term vector refers to

• A. the enzyme that cuts $DNA$ into restriction fragments
• B. the sticky end of a $DNA$ fragment
• C. a plasmid used to transfer $DNA$ into a living cell
• D. a $DNA$ fragment which carries only $ori$ gene.

Answer 1

Answer: (C)

a plasmid used to transfer $DNA$ into a living cell

Answer 2

The $DNA$ used as a carrier for transferring a fragment of foreign $DNA$ into a suitable host is called vehicle $DNA$ or cloning vector or gene carrier. When desired gene is introduced into a vector, recombinant $DNA$ is formed. Vectors may be plasmids, bacteriophages, cosmids, phagemids, Yeast Artificial Chromosomes $(YACs)$, Bacterial Artificial Chromosomes $(BACs)$, transposons, viruses, etc.