Question
Biology Question on biotechnology
In plant biotechnology, PEG is used in
A
protoplast isolation
B
cell culture preparation
C
protoplast fusion
D
hardening
Answer
protoplast fusion
Explanation
Solution
Somatic hybridization involves the fusion of protoplasts of two different species which resulted in hybrid. Naked protoplasts are obtained by dissolution of their cell walls by the mecerating enzymes such as pectinase and cellulase. Fusion of protoplasts from two different varieties can be enhanced by treating with polyethylene glycol (PEG) in presence of high voltage electric current.