Question
Biology Question on biotechnology
In Lederberg's replica plating experiment what shall be used to obtain streptomycin resistant strain
A
minimal medium and streptomycin
B
complete medium and streptomycin
C
only minimal medium
D
only complete medium.
Answer
minimal medium and streptomycin
Explanation
Solution
If streptomycin resistant mutant are to be obtained, material should be allowed to grow on medium lacking streptomycin so that both mutant and wild types may grow. These colonies are imprinted on petriplates to form the master pattern and other plates having streptomycin can then be pressed on velveteen to get an impression. The plate now containing only mutants for streptomycin resistance will grow.